General CRISPR RNP Transfection Guidelines

Choosing the right delivery reagent is critical for transfection and gene editing efficiency. See our recommendations in Table 1. For more information on transfection reagents, see thermofisher.com/transfection.
Cell line–specific conditions are provided in the tables: Cell line–specific transfection conditions using the Lipofectamine CRISPRMAX Transfection Reagent and Cell line–specific CRISPR RNP electroporation conditions using Neon System.

Cas9 format	Transfection reagent*	Electroporation**
TrueCut Cas9 Protein v2 + gRNA	Lipofectamine CRISPRMAX Cas9 Transfection Reagent	For maximum efficiency in difficult-to-transfect cell types, use the Neon Transfection System
* For best results, we recommend that you transfect your cells with TrueCut Cas9 Protein v2 + TrueGuide Synthetic gRNA (crRNA:tracrRNA duplex or sgRNA) using the Lipofectamine CRISPRMAX Cas9 Transfection Reagent. ** Use the Neon Transfection System 10 μL Kit (Cat. No. MPK1025).